NEW PAPER: cell culture for lizards
In a new paper that both develops a method and evaluates it, John Smout publishes on primary cell culture from lizard oviduct or uterus tissue Published in Experimental Cell Research This was a collaboration between our lab and the cell culture expertise of Mark McLaughlin at the Vet School. It is part of John's PhD work, which was funded from the University of Glasgow MVLS-DTP Abstract Reproduction by egg-laying (oviparity) or live-bearing (viviparity) is a genetically determined trait fundamental to the biology of amniotes. Squamates are an emerging model for the genetics of reproductive mode yet lack cell culture models valuable for exploring molecular mechanisms. Here, we report a novel primary culture model for reproductive biology: cell cultures derived from the oviduct tissues (infundibulum, uterus and vagina) of oviparous and viviparous common lizards (Lacertidae: Zootoca vivipara) . We maintained and expanded these cultures for over 100 days, including repeated subcultu